The CC17 protein with a His tag was purified using the Nickel resin column (Bio-Rad, Hercules, CA, USA). The bound protein of CC17 on the resin was eluted by 150 ...
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(Ifn-γf: 543 Forward: 5'-ATGAACGCTACACACTGCATC-3' 544 Reverse: 5'-CCATCCTTTTGCCAGTTCCTC-3' 545 546 Quantification of cytokines and insulin production by enzyme-l...